DNA Extraction from Teeth

1. Before starting, thoroughly clean (brush) the teeth with a soap and rinse with distilled water. Take care to clean each tooth separately, rinsing well after cleaning.
2. Soak each tooth in bleach for 30 minutes.
3. Remove and wash with distilled water, removing all traces of bleach.
4. Place in a fume hood with airflow turned on, and illuminate with ultraviolet light for 30 minutes.
5. Place each tooth into a 15 mL Falcon tube, and cover with 0.5 M EDTA.
6. Leave at 55°C for 6 to 7 days, periodically mixing by inversion to resuspend settled content.
7. Add proteinase K (for a sliver of tooth 10 mL for an entire tooth 30 mL) and DTT (half the amount of proteinase K) and leave incubating overnight at 55°C.
8. Add equal volume of RNAse as of DTT, and let incubate at 37°C for 15 to 60 minutes.
9. Remove 500 mL of the digest, and continue with phenol chloroform extraction (Sambrook et al. 1989).

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